Development and Validation of Stability Indicating Rp-hplc Assay Method of Zolmitriptan in Pure and Pharmaceutical Dosage Forms K.parameswara Rao, G.v.ramana

Article Info: Received: 17/09/2014 Revised on : 25/09/2014 Accepted on: 28/09/2014 ABSTRACT A simple, accurate and stability-indicating reversed phase high performance liquid chromatographic method was developed and validated for the determination of Zolmitriptan and using a Altima C18 (150 x 4.6 mm), 5μm column using a mobile phase of phosphate buffer pH 2.5 and acetonitrile in the proportion of 85:15. The retention time of zolmitriptan were found to be 4.522min respectively. Linearity was established for Zolmitriptan in the range of 5.104 to 30.626μg/ml. The percentage recovery of Zolmitriptan was found to be in the range of 98.50-100% respectively. The selected drug were subjected to acid, alkali, oxidation, dry heat and UV degradation and these degradation studies revealed that this method can be successfully employed for stability indicating method for routine analysis of Zolmitriptan in pure and formulations.